Part:BBa_K777109:Experience

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Team Glasgow 2014 Experience

We made our own version of a fliC biobrick K1463600. Our version had the MG1655 fliC sequence instead of the DH10B sequence fliC of this biobrick. Our fliC biobrick restored swimming in a fliC deletion mutant of E. coli.
see BBa_K1463600 for more details.

Flic Motility Histogram

Figure 2 - FliC Motility Histogram The promoters indicated in the histogram were used to drive the fliC biobrick K1463600 with the RBS B0034. Plasmids containing these constructs were used to complement a chromosomal fliC mutation in the stain DS941 delta fliC. The diameter of swimming in a 16 hour swarm assay at 37 degrees is shown. The error bars indicate the range or results obtained in two repeats of the experiment.

User Reviews

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