Coding

Part:BBa_K777109:Experience

Designed by: Flash Coli   Group: iGEM12_Goettingen   (2012-09-18)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K777109

Team Glasgow 2014 Experience

We made our own version of a fliC biobrick K1463600. Our version had the MG1655 fliC sequence instead of the DH10B sequence fliC of this biobrick. Our fliC biobrick restored swimming in a fliC deletion mutant of E. coli.
see BBa_K1463600 for more details.

Flic Motility Histogram

GU_Figure_2_Motility_histogram.png
Figure 2 - FliC Motility Histogram The promoters indicated in the histogram were used to drive the fliC biobrick K1463600 with the RBS B0034. Plasmids containing these constructs were used to complement a chromosomal fliC mutation in the stain DS941 delta fliC. The diameter of swimming in a 16 hour swarm assay at 37 degrees is shown. The error bars indicate the range or results obtained in two repeats of the experiment.

User Reviews

UNIQd314cbdf03dea835-partinfo-00000000-QINU UNIQd314cbdf03dea835-partinfo-00000001-QINU